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AMB Express
Fig. 4 | AMB Express

Fig. 4

From: Evaluation of an oxygen‐dependent self‐inducible surfactin synthesis in B. subtilis by substitution of native promoter PsrfA by anaerobically active PnarG and PnasD

Fig. 4

Exemplary cultivation results of fed-batch bioreactor cultivations employing strains B. subtilis KM1016 (PsrfA-lacZ) (a, c) and B. subtilis MG14 (PsrfA::PnasD; amyE::[PnasD-lacZ, spcR]) (b, d). Displayed are absolute values of cell dry weight (CDW), glucose, surfactin (all [g]), nitrate, nitrite and ammonium (all [mmol]) (a, b), as well as the time course of OD600, Miller units as determined by the Miller Assay to determine the expression of PsrfA and PnasD, and pO2 [%] (c, d)

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