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Table 4 Comparison of CGP synthesis in C. glutamicum strains for cadaverine production

From: Incorporation of alternative amino acids into cyanophycin by different cyanophycin synthetases heterologously expressed in Corynebacterium glutamicum

Strains DW [g/l] Cyanophycin
   Solubility CGP content [%DW] Amino acid composition [mol%] *
Asp Arg Lys Glu Orn Cit Cad
DM1279 pEC-XT99A::ldcC
 pVWEx1::cphA6308Δ1 13.3 ± 0.1 Insoluble 3.5 ± 0.9 46.6 46.2 6.2 0.4 0.1 0.5 0.0
Soluble 3.5 ± 1.4 44.1 38.0 13.6 1.6 2.2 0.5 0.0
 pVWEx1::cphA6308Δ1_C595S 12.7 ± 0.3 Insoluble 3.2 ± 1.2 47.4 45.8 5.9 0.4 0.1 0.4 0.0
Soluble 1.5 ± 1.2 44.2 37.1 16.0 1.9 0.0 0.8 0.0
 pVWEx1::cphADh 9.2 ± 0.1 Insoluble 0.0 ± 0.0
Soluble 20.4 ± 0.7 44.1 41.9 11.0 2.2 0.5 0.3 0.0
  1. The strains were cultivated for 72 h in 100 ml CGXII medium in 500 ml Erlenmeyer flasks with two baffles at 130 rpm and 30 °C. The cultures were inoculated with an OD600 of about 0.2 and after 4 h IPTG was added. The cells dry mass was determined and the contained cyanophycin was isolated and analyzed. The values indicate the mean and standard deviation of at least three independent cultivations
  2. * The values have been rounded to one decimal place