Skip to main content
AMB Express

Table 4 Comparison of CGP synthesis in C. glutamicum strains for cadaverine production

From: Incorporation of alternative amino acids into cyanophycin by different cyanophycin synthetases heterologously expressed in Corynebacterium glutamicum

Strains

DW [g/l]

Cyanophycin

  

Solubility

CGP content [%DW]

Amino acid composition [mol%] *

Asp

Arg

Lys

Glu

Orn

Cit

Cad

DM1279 pEC-XT99A::ldcC

 pVWEx1::cphA6308Δ1

13.3 ± 0.1

Insoluble

3.5 ± 0.9

46.6

46.2

6.2

0.4

0.1

0.5

0.0

Soluble

3.5 ± 1.4

44.1

38.0

13.6

1.6

2.2

0.5

0.0

 pVWEx1::cphA6308Δ1_C595S

12.7 ± 0.3

Insoluble

3.2 ± 1.2

47.4

45.8

5.9

0.4

0.1

0.4

0.0

Soluble

1.5 ± 1.2

44.2

37.1

16.0

1.9

0.0

0.8

0.0

 pVWEx1::cphADh

9.2 ± 0.1

Insoluble

0.0 ± 0.0

–

–

–

–

–

–

–

Soluble

20.4 ± 0.7

44.1

41.9

11.0

2.2

0.5

0.3

0.0

  1. The strains were cultivated for 72 h in 100 ml CGXII medium in 500 ml Erlenmeyer flasks with two baffles at 130 rpm and 30 °C. The cultures were inoculated with an OD600 of about 0.2 and after 4 h IPTG was added. The cells dry mass was determined and the contained cyanophycin was isolated and analyzed. The values indicate the mean and standard deviation of at least three independent cultivations
  2. * The values have been rounded to one decimal place
Back to article page