Table 1 Strains and plasmids used in this study

Strains

 E. coli TOP10

F- mcrA Δ(mrr-hsd RMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 ga/U ga/

K rpsL (Str^R) endA1 nupG

Invitrogen, Carlsbad, USA

 C. glutamicum ATCC 13,032

Wild type, Biotin-auxotroph

DSMZ, Braunschweig, Germany

 C. glutamicum DM1729

ATCC 13032 pyc ^P458S, hom ^V59A, lysC ^T311I

Georgi et al. (2005)

 C. glutamicum ORN2(P_{gdh 4})

ATCC 13032 with in-frame deletion of prophages CGP1 (cg1507-cg1524), CGP2 (cg1746-cg1752), and CGP3 (cg1890-cg2071), ΔargFRG,

P_gdh4: − 35: TTGCCA − 10: TATAAT

Jensen et al. (2015)

Plasmids

 pVWEx1

P_tac, lacI ^q, Km^R

Peters-Wendisch et al. (2001)

 pVWEx1::cphA₆₃₀₈ Δ1

pVWEx1 with cphA₆₃₀₈ Δ1 (PstI/XbaI)

Wiefel et al. (2019a)

 pVWEx1::cphA₆₃₀₈ Δ1_C595S

pVWEx1 with cphA₆₃₀₈ Δ1_C595S (PstI/XbaI)

This study

 pVWEx1::cphA_Dh

pVWEx1 with cphA_Dh (SdaI/XbaI)

Wiefel et al. (2019a)

 pEC-XT99A::ldcC

P_trc, lacI ^q, pGA1 OriV_C:g., ldcC from E. coli K12, Tet^R

Sgobba et al. (2018)