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Table 1 Strains and plasmids used in this study

From: Incorporation of alternative amino acids into cyanophycin by different cyanophycin synthetases heterologously expressed in Corynebacterium glutamicum

Strain or plasmid Relevant characteristics Reference or source
Strains
 E. coli TOP10 F- mcrA Δ(mrr-hsd RMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 ga/U ga/
K rpsL (StrR) endA1 nupG
Invitrogen, Carlsbad, USA
 C. glutamicum ATCC 13,032 Wild type, Biotin-auxotroph DSMZ, Braunschweig, Germany
 C. glutamicum DM1729 ATCC 13032 pyc P458S, hom V59A, lysC T311I Georgi et al. (2005)
 C. glutamicum ORN2(Pgdh 4) ATCC 13032 with in-frame deletion of prophages CGP1 (cg1507-cg1524), CGP2 (cg1746-cg1752), and CGP3 (cg1890-cg2071), ΔargFRG,
Pgdh4: − 35: TTGCCA − 10: TATAAT
Jensen et al. (2015)
Plasmids
 pVWEx1 Ptac, lacI q, KmR Peters-Wendisch et al. (2001)
 pVWEx1::cphA6308 Δ1 pVWEx1 with cphA6308 Δ1 (PstI/XbaI) Wiefel et al. (2019a)
 pVWEx1::cphA6308 Δ1_C595S pVWEx1 with cphA6308 Δ1_C595S (PstI/XbaI) This study
 pVWEx1::cphADh pVWEx1 with cphADh (SdaI/XbaI) Wiefel et al. (2019a)
 pEC-XT99A::ldcC Ptrc, lacI q, pGA1 OriVC:g., ldcC from E. coli K12, TetR Sgobba et al. (2018)
  1. KmR kanamycin resistance, TetR tetracycline resistance