Fig. 4

A. oryzae hyphal growth and differentiation under different stress factors for 72 h. a The phenotypes of A. oryzae under temperature and salinity stress. (a–e) Phenotypes of A. oryzae exposed to different temperature stresses (22, 25, 30, 35, and 42 ℃ from left to right). (f-j) 0.0, 5.0, 10.0, 12.5, and 15.0 g/100 mL NaCl was used for salinity stress. b and c Colony diameter was determined by measuring diameter under different stress conditions. d and e Spore numbers were counted under temperature and salinity stresses. The spores were collected from colonies and suspended in sterile distilled water to obtain the spore suspension, and then the spore numbers were counted using a hemocytometer. The optimum growth temperature of A. oryzae (30 ℃), was used as the control temperature in the experiment. The PDA medium without NaCl used as the control in the salt treatment experiments. Results represent the average of three repetitions ± SEM (n = 3). Different letters in the bar chart represent significant differences (p < 0.01, Duncan's multiple range test); the same letters in the bar chart represent absence of significant difference when compared to the control