Table 1 Recovery and purity of Δ1-11-G129R-hPRL after each purification step
From: Periplasmic synthesis and purification of the human prolactin antagonist Δ1-11-G129R-hPRL
Purification step | Δ1-11-G129R-hPRL (μg) | Recovery (%) | Purity (%) |
|---|---|---|---|
Periplasmic fluid | 3600 | – | – |
IMAC (Hisprep FF) | 1160 | 32.2 | 65.7 |
SEC (Sephacryl S100) | 884 | 76.2 | 96.5 |