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Table 4 The design and result of one-factor-at-time for optimization of biomass production in bioreactor

From: Growth optimization and identification of an ω-transaminase by a novel native PAGE activity staining method in a Bacillus sp. strain BaH isolated from Iranian soil

Run Variable factor Constant factor Fermenters
[Absorbance in 600nm and AcPhe concentration mM (in parentheses)]
F1 F2 F3 F4 F5 F6
1 Stirrer speed (rpm) Aeration
(1.5 vvm)
200 rpm
(1.8, 0.25 mM)
600 rpm
(5.2, 1.9 mM)
1200 rpm
( 2.1, 0.3 mM)
2 400 rpm
(2.4, 1.7 mM)
600 rpm
(5.1, 2.1 mM)
800 rpm
(4.7, 1.4 mM)
3 Aeration (vvm) Stirrer speed (600 rpm) 0.5 vvm
(5.3, 3.5 mM)
1 vvm
(5.2, 2.6 mM)
1.5 vvm
(5.4, 2.3 mM)