Table 4 The design and result of one-factor-at-time for optimization of biomass production in bioreactor
Run | Variable factor | Constant factor | Fermenters [Absorbance in 600nm and AcPhe concentration mM (in parentheses)] | |||||
|---|---|---|---|---|---|---|---|---|
F1 | F2 | F3 | F4 | F5 | F6 | |||
1 | Stirrer speed (rpm) | Aeration (1.5 vvm) | 200 rpm (1.8, 0.25 mM) | 600 rpm (5.2, 1.9 mM) | 1200 rpm ( 2.1, 0.3 mM) | |||
2 | 400 rpm (2.4, 1.7 mM) | 600 rpm (5.1, 2.1 mM) | 800 rpm (4.7, 1.4 mM) | |||||
3 | Aeration (vvm) | Stirrer speed (600 rpm) | 0.5 vvm (5.3, 3.5 mM) | 1 vvm (5.2, 2.6 mM) | 1.5 vvm (5.4, 2.3 mM) | |||