Growth optimization and identification of an ω-transaminase by a novel native PAGE activity staining method in a Bacillus sp. strain BaH isolated from Iranian soil

AMB Express

Table 2 Design of experiments for the optimization of medium for biomass and ω-TA production in Bacillus sp. strain BaH, biomass production and AcPhe concentration were considered as a response

Run	A (°C)	B (rpm)	C (pH)	Biomass production (OD_600nm)	AcPhe (mM)
1	33	160	7.7	0.18	0.04
2	33	160	8.8	0.6	0.03
3	33	160	7.7	0.25	0.04
4	28	120	7	0.27	0.67
5	33	160	7.7	0.19	0.03
6	33	216	7.7	0.09	0.01
7	28	200	8.5	0.39	0.01
8	40	160	7.7	1.61	0.03
9	33	160	7.7	0.17	0.19
10	33	100	7.7	0.56	0.01
11	33	160	6.7	3.24	2.76
12	38	200	7	5.65	3.29
13	33	160	7.7	1.92	0.66
14	26	160	7.7	0.22	0.02
15	38	120	8.5	0.94	0.01