Skip to main content

Table 2 Design of experiments for the optimization of medium for biomass and ω-TA production in Bacillus sp. strain BaH, biomass production and AcPhe concentration were considered as a response

From: Growth optimization and identification of an ω-transaminase by a novel native PAGE activity staining method in a Bacillus sp. strain BaH isolated from Iranian soil

Run A (°C) B (rpm) C (pH) Biomass production (OD600nm) AcPhe
(mM)
1 33 160 7.7 0.18 0.04
2 33 160 8.8 0.6 0.03
3 33 160 7.7 0.25 0.04
4 28 120 7 0.27 0.67
5 33 160 7.7 0.19 0.03
6 33 216 7.7 0.09 0.01
7 28 200 8.5 0.39 0.01
8 40 160 7.7 1.61 0.03
9 33 160 7.7 0.17 0.19
10 33 100 7.7 0.56 0.01
11 33 160 6.7 3.24 2.76
12 38 200 7 5.65 3.29
13 33 160 7.7 1.92 0.66
14 26 160 7.7 0.22 0.02
15 38 120 8.5 0.94 0.01