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AMB Express
Fig. 5 | AMB Express

Fig. 5

From: Growth optimization and identification of an ω-transaminase by a novel native PAGE activity staining method in a Bacillus sp. strain BaH isolated from Iranian soil

Fig. 5

Colorimetric assay for localization of ω-TAs on native polyacrylamide gel. Crude extracts of a 3FCR, overexpressed in E. coli. b Bacillus sp. strain BaH, each gel from left to right, containing Coomassie brilliant blue staining (as described in method section) and activity staining assay was carried out in 50 mM HEPES buffer, pH 7.5 with OXD (5 mM), pyruvate (7.5 mM) DMSO (5 %) and PLP (1 mM). The numbers indicating the protein marker bands resemble the order of the bands when used for SDS-PAGE according to the manufacturer’s manual and cannot be used for molecular weight determination in native gels. However, we used this pre-stained marker to demonstrate the color development and the specific staining of the ω-TA band

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