Fig. 5
From: Numaswitch: an efficient high-titer expression platform to produce peptides and small proteins
Numaswitch applied to produce Teriparatide a SDS-PAGE analysis of different process steps; E. coli cells from high cell density fermentation (E), solubilized Switchtag Teriparatide IBs (S), renaturation reaction (R), TEV protease cleavage (C1: crude, C2, cleared) and produced Teriparatide after CEX, RP FLASH purification and TFA/acetate exchange (P). M: Molecular weight marker with shown masses. b HPLC/MS analysis of purified Teriparatide revealed > 99.6% purity of lyophilized Teriparatide