Fig. 3

Renaturation efficiencies and TEV protease cleavage of HlyA1 fusions. a Renaturation efficiencies (%) of HlyA1 fusions in the presence or absence of 10 mM Ca²⁺ ions. 100% renaturation efficiency corresponds to the adjusted protein concentration for the renaturation reaction. Error bars indicate the SD (n = 3). b SDS-PAGE analysis (Coomassie-stained) of renatured HlyA1 fusions prior to and after TEV protease cleavage. Arrows indicate the HlyA1 fusions after renaturation (R) and HlyA1 after TEV protease cleavage (C). Signals were found for each target but Linaclotide. Due to visualization limits of Coomassie staining, the location is highlighted (*). Release of targets was further assessed by RP-HPLC analysis (Additional file 1: Fig. S3)