Improvement and prediction of secondary metabolites production under yeast extract elicitation of Azadirachta indica cell suspension culture using response surface methodology

AMB Express

Table 4 Analysis of variance of azadirachtin, mevalonic acid and squalene accumulation and production in the central composite design under influence of yeast extract and sampling times

Source	df	Mean of square
Source	df	Azadirachtin accumulation	Azadirachtin production	Mevalonic acid accumulation	Mevalonic acid production	Squalene accumulation	Squalene production
Model	5	6.95^s	5058.59^s	0.02^s	5.46^s	0.004^s	1.33^s
A: Yeast extract	1	0.72^ns	46.34^ns	0.04^s	0.001^ns	0.003^s	0.75^s
B: Sampling time	1	22.31^s	17,458.30^s	0.005^ns	0.005^ns	0.01^s	3.55^s
AB	1	4.76^s	502.37^ns	0.003^ns	0.27^ns	0.003^s	0.93^s
A²	1	4.00^s	7206.08^s	0.02^s	0.07^ns	0.001^ns	0.12^ns
B²	1	1.18^ns	241.38^ns	0.03^s	24.06^s	0.004^s	1.39^s
Residual	7	0.22	227.39	0.002	0.56	0.0002	0.06
Lack of fit	3	0.41^ns	436.22^ns	0.001^ns	0.28^ns	0.0003^ns	0.10^ns
Pure error	4	0.08	70.77	0.002	0.76	0.0001	0.02
R² (%)		95.76	94.08	91.67	87.53	94.20	94.48
R₂^adj (%)		92.73	89.85	85.71	78.63	90.05	90.55
Adeq precision		18.94	14.89	15.79	8.65	14.49	15.84