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Table 4 Analysis of variance of azadirachtin, mevalonic acid and squalene accumulation and production in the central composite design under influence of yeast extract and sampling times

From: Improvement and prediction of secondary metabolites production under yeast extract elicitation of Azadirachta indica cell suspension culture using response surface methodology

Source df Mean of square
Azadirachtin accumulation Azadirachtin production Mevalonic acid accumulation Mevalonic acid production Squalene accumulation Squalene production
Model 5 6.95s 5058.59s 0.02s 5.46s 0.004s 1.33s
A: Yeast extract 1 0.72ns 46.34ns 0.04s 0.001ns 0.003s 0.75s
B: Sampling time 1 22.31s 17,458.30s 0.005ns 0.005ns 0.01s 3.55s
AB 1 4.76s 502.37ns 0.003ns 0.27ns 0.003s 0.93s
A2 1 4.00s 7206.08s 0.02s 0.07ns 0.001ns 0.12ns
B2 1 1.18ns 241.38ns 0.03s 24.06s 0.004s 1.39s
Residual 7 0.22 227.39 0.002 0.56 0.0002 0.06
Lack of fit 3 0.41ns 436.22ns 0.001ns 0.28ns 0.0003ns 0.10ns
Pure error 4 0.08 70.77 0.002 0.76 0.0001 0.02
R2 (%)   95.76 94.08 91.67 87.53 94.20 94.48
R2adj (%)   92.73 89.85 85.71 78.63 90.05 90.55
Adeq precision   18.94 14.89 15.79 8.65 14.49 15.84
  1. s and nssignificant and non-significant, respectively