Improvement and prediction of secondary metabolites production under yeast extract elicitation of Azadirachta indica cell suspension culture using response surface methodology

AMB Express

Table 3 Analysis of variance of the effect of yeast extract and sampling times on measured indices in cell suspension culture of neem

S.O.V		Yeast extract (YE)	Sampling time (ST)	YE × ST	Error	CV (%)
df		5	5	25	72	CV (%)
Mean of square	Fresh cell weight	93,350.82**	179,438.79**	33,712.14**	2256.49	16.70
	Dry cell weight	61.88**	229.58**	24.07**	1.35	9.27
	Azadirachtin accumulation	72.18**	37.009**	11.93**	0.60	11.23
	Azadirachtin production	10,274.50**	27,632.98**	3433.52**	115.54	13.24
	Mevalonic acid accumulation	0.88**	1.47**	0.51**	0.007	24.67
	Mevalonic acid production	177.95**	314.43**	108.41**	1.51	26.89
	Squalene accumulation	0.006**	0.02**	0.006**	0.00003	12.55
	Squalene production	2.30**	6.87**	1.98**	0.02	21.88