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Table 2 Experimental and predicted values for sequalene accumulation and production optimized with central composite design (CCD)

From: Improvement and prediction of secondary metabolites production under yeast extract elicitation of Azadirachta indica cell suspension culture using response surface methodology

Experiment Yeast extract (mg/L) A Sampling time (day) (B) Squalene accumulation (mg/g DW) Squalene production (mg/L)
Actual value Predicted value Actual value Predicted value
1 100 (0) 8 (0) 0.01 0.02 0.12 0.22
2 100 (0) 8 (0) 0.01 0.02 0.11 0.22
3 100 (0) 4 (− 2) 0.13 0.13 2.21 2.29
4 100 (0) 12 (+ 2) 0.01 0.01 0.09 0.11
5 100 (0) 8 (0) 0.01 0.02 0.12 0.22
6 50 (− 1) 6 (− 1) 0.14 0.11 2.15 1.81
7 200 (+ 2) 8 (0) 0.02 0.01 0.16 0.00
8 150 (+ 1) 6 (− 1) 0.03 0.03 0.2 0.34
9 100 (0) 8 (0) 0.01 0.02 0.11 0.22
10 150 (+ 1) 10 (+ 1) 0.01 0.02 0.09 0.22
11 100 (0) 8 (0) 0.03 0.02 0.41 0.22
12 0 (− 2) 8 (0) 0.06 0.08 0.75 1.01
13 50 (− 1) 10 (+ 1) 0.01 0.00 0.03 0.00
  1. Bold values indicated the highest amount