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Table 2 Experimental and predicted values for sequalene accumulation and production optimized with central composite design (CCD)

From: Improvement and prediction of secondary metabolites production under yeast extract elicitation of Azadirachta indica cell suspension culture using response surface methodology

Experiment

Yeast extract (mg/L) A

Sampling time (day) (B)

Squalene accumulation (mg/g DW)

Squalene production (mg/L)

Actual value

Predicted value

Actual value

Predicted value

1

100 (0)

8 (0)

0.01

0.02

0.12

0.22

2

100 (0)

8 (0)

0.01

0.02

0.11

0.22

3

100 (0)

4 (− 2)

0.13

0.13

2.21

2.29

4

100 (0)

12 (+ 2)

0.01

0.01

0.09

0.11

5

100 (0)

8 (0)

0.01

0.02

0.12

0.22

6

50 (− 1)

6 (− 1)

0.14

0.11

2.15

1.81

7

200 (+ 2)

8 (0)

0.02

0.01

0.16

0.00

8

150 (+ 1)

6 (− 1)

0.03

0.03

0.2

0.34

9

100 (0)

8 (0)

0.01

0.02

0.11

0.22

10

150 (+ 1)

10 (+ 1)

0.01

0.02

0.09

0.22

11

100 (0)

8 (0)

0.03

0.02

0.41

0.22

12

0 (− 2)

8 (0)

0.06

0.08

0.75

1.01

13

50 (− 1)

10 (+ 1)

0.01

0.00

0.03

0.00

  1. Bold values indicated the highest amount