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Table 1 Experimental and predicted values for azadirachtin and mevalonic acid accumulation and production optimized with central composite design (CCD)

From: Improvement and prediction of secondary metabolites production under yeast extract elicitation of Azadirachta indica cell suspension culture using response surface methodology

Experiment Yeast extract (mg/L) A Sampling time (day) (B) Azadirachtin accumulation (mg/g DW) Azadirachtin production (mg/L) Mevalonic acid accumulation (mg/g DW) Mevalonic acid production (mg/L)
Actual value Predicted value Actual value Predicted value Actual value Predicted value Actual value Predicted value
1 50 (− 1) 4 (− 1) 6.10 6.30 160.56 147.73 0.12 0.14 3.63 4.02
2 100 (0) 2 (− 2) 9.89 10.10 170.37 184.63 0.09 0.07 1.29 1.12
3 50 (− 1) 8 (+ 1) 5.16 5.75 32.44 49.03 0.25 0.24 4.16 4.58
4 0 (− 2) 6 (0) 4.70 4.30 47.88 46.46 0.26 0.26 5.71 5.50
5 100 (0) 10 (+ 2) 4.83 4.64 47.22 32.05 0.14 0.15 1.41 1.20
6 100 (0) 6 (0) 6.50 6.46 119.68 121.32 0.28 0.25 5.12 5.26
7 100 (0) 6 (0) 6.05 4.46 108.61 121.32 0.28 0.25 4.97 5.26
8 150 (+ 1) 4 (− 1) 9.59 8.97 144.01 129.24 0.27 0.31 4.19 4.52
9 100 (0) 6 (0) 6.83 6.46 122.59 121.32 0.278 0.25 4.97 5.26
10 200 (+ 2) 6 (0) 4.87 5.28 53.81 54.32 0.51 0.49 5.62 5.46
11 100 (0) 6 (0) 6.46 6.46 126.96 121.32 0.28 0.25 5.02 5.26
12 100 (0) 6 (0) 6.44 6.46 130.60 121.32 0.18 0.25 6.97 5.26
13 150 (+ 1) 8 (+ 1) 4.29 4.06 60.72 75.37 0.28 0.29 3.67 4.04
  1. Bold values indicated the highest amount