Table 1 Experimental and predicted values for azadirachtin and mevalonic acid accumulation and production optimized with central composite design (CCD)
Experiment | Yeast extract (mg/L) A | Sampling time (day) (B) | Azadirachtin accumulation (mg/g DW) | Azadirachtin production (mg/L) | Mevalonic acid accumulation (mg/g DW) | Mevalonic acid production (mg/L) | ||||
|---|---|---|---|---|---|---|---|---|---|---|
Actual value | Predicted value | Actual value | Predicted value | Actual value | Predicted value | Actual value | Predicted value | |||
1 | 50 (− 1) | 4 (− 1) | 6.10 | 6.30 | 160.56 | 147.73 | 0.12 | 0.14 | 3.63 | 4.02 |
2 | 100 (0) | 2 (− 2) | 9.89 | 10.10 | 170.37 | 184.63 | 0.09 | 0.07 | 1.29 | 1.12 |
3 | 50 (− 1) | 8 (+ 1) | 5.16 | 5.75 | 32.44 | 49.03 | 0.25 | 0.24 | 4.16 | 4.58 |
4 | 0 (− 2) | 6 (0) | 4.70 | 4.30 | 47.88 | 46.46 | 0.26 | 0.26 | 5.71 | 5.50 |
5 | 100 (0) | 10 (+ 2) | 4.83 | 4.64 | 47.22 | 32.05 | 0.14 | 0.15 | 1.41 | 1.20 |
6 | 100 (0) | 6 (0) | 6.50 | 6.46 | 119.68 | 121.32 | 0.28 | 0.25 | 5.12 | 5.26 |
7 | 100 (0) | 6 (0) | 6.05 | 4.46 | 108.61 | 121.32 | 0.28 | 0.25 | 4.97 | 5.26 |
8 | 150 (+ 1) | 4 (− 1) | 9.59 | 8.97 | 144.01 | 129.24 | 0.27 | 0.31 | 4.19 | 4.52 |
9 | 100 (0) | 6 (0) | 6.83 | 6.46 | 122.59 | 121.32 | 0.278 | 0.25 | 4.97 | 5.26 |
10 | 200 (+ 2) | 6 (0) | 4.87 | 5.28 | 53.81 | 54.32 | 0.51 | 0.49 | 5.62 | 5.46 |
11 | 100 (0) | 6 (0) | 6.46 | 6.46 | 126.96 | 121.32 | 0.28 | 0.25 | 5.02 | 5.26 |
12 | 100 (0) | 6 (0) | 6.44 | 6.46 | 130.60 | 121.32 | 0.18 | 0.25 | 6.97 | 5.26 |
13 | 150 (+ 1) | 8 (+ 1) | 4.29 | 4.06 | 60.72 | 75.37 | 0.28 | 0.29 | 3.67 | 4.04 |