Table 4 Central composite design: experimental procedure and variables
Two-stage fermentation | ||||
|---|---|---|---|---|
Pre-growth stage (24 h) in the presense of 1.0 g/L FeCl3 Biomass morphology: dispersed mycelium | ||||
Lipase production stage (72 h), initial biomass concentration: 15 gwet weight/L | ||||
Trial number | Input variables | Response variable | ||
N:C ratio | FeCl3 | Olive oil | Lipase activity (U/L) | |
CP | 0 | 0 | 0 | 153.73 ± 2.17 |
CP | 0 | 0 | 0 | 82,94 ± 0.29 |
CP | 0 | 0 | 0 | 219.61 ± 6.50 |
CP | 0 | 0 | 0 | 191.01 ± 5.78 |
CP | 0 | 0 | 0 | 238.76 ± 14.80 |
CP | 0 | 0 | 0 | 239.78 ± 5.42 |
1 | + 1 | + 1 | − 1 | 3.33 ± 0.21 |
2 | − 1 | − 1 | + 1 | 294.90 ± 25.94 |
3 | 0 | 0 | − α | 89.59 ± 1.73 |
4 | − 1 | + 1 | + 1 | 324.57 ± 19.86 |
5 | + 1 | − 1 | − 1 | 20.13 ± 0.87 |
6 | + 1 | − 1 | + 1 | 6.56 ± 4.27 |
7 | 0 | + α | 0 | 315.63 ± 32.50 |
8 | 0 | 0 | + α | 251.28 ± 10.47 |
9 | − 1 | + 1 | − 1 | 135.60 ± 10.11 |
10 | + 1 | + 1 | + 1 | 170.84 ± 7.95 |
11 | 0 | − α | 0 | 276.05 ± 48.76 |
12 | + α | 0 | 0 | 1.57 ± 0.41 |
13 | − α | 0 | 0 | 30.16 ± 11.66 |
14 | − 1 | − 1 | − 1 | 242.98 ± 30.52 |