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Table 4 Central composite design: experimental procedure and variables

From: Orchestration an extracellular lipase production from Aspergillus niger MYA 135: biomass morphology and fungal physiology

Two-stage fermentation
Pre-growth stage (24 h) in the presense of 1.0 g/L FeCl3
Biomass morphology: dispersed mycelium
Lipase production stage (72 h), initial biomass concentration: 15 gwet weight/L
Trial number Input variables Response variable
N:C ratio FeCl3 Olive oil Lipase activity (U/L)
CP 0 0 0 153.73 ± 2.17
CP 0 0 0 82,94 ± 0.29
CP 0 0 0 219.61 ± 6.50
CP 0 0 0 191.01 ± 5.78
CP 0 0 0 238.76 ± 14.80
CP 0 0 0 239.78 ± 5.42
1 + 1 + 1 − 1 3.33 ± 0.21
2 − 1 − 1 + 1 294.90 ± 25.94
3 0 0 − α 89.59 ± 1.73
4 − 1 + 1 + 1 324.57 ± 19.86
5 + 1 − 1 − 1 20.13 ± 0.87
6 + 1 − 1 + 1 6.56 ± 4.27
7 0  + α 0 315.63 ± 32.50
8 0 0 + α 251.28 ± 10.47
9 − 1 + 1 − 1 135.60 ± 10.11
10 + 1 + 1 + 1 170.84 ± 7.95
11 0 − α 0 276.05 ± 48.76
12 + α 0 0 1.57 ± 0.41
13 − α 0 0 30.16 ± 11.66
14 − 1 − 1 − 1 242.98 ± 30.52