|
Time (h)
|
CP1 or CPS1
|
CP2 or CPS2
|
CP3 or CPS3
|
CP4 or CPS4
|
CP5 or CPS5
|
---|
Intestinal epithelium cells
|
CP
|
12
|
198.25 ± 10.53b
|
231.58 ± 5.26c
|
157.89 ± 5.26a
|
145.61 ± 7.02a
|
207.02 ± 3.51b
|
24
|
130.23 ± 9.30a
|
120.93 ± 11.16ab
|
90.70 ± 8.10d
|
109.3 ± 7.67c
|
109.30 ± 6.60bc
|
48
|
84.78 ± 8.70a
|
80.43 ± 4.35a
|
60.87 ± 3.04b
|
47.83 ± 4.35c
|
50.00 ± 3.52c
|
CPS
|
12
|
116.85 ± 5.01bc
|
113.88 ± 4.87c
|
105.39 ± 1.52d
|
136.13 ± 1.59 a
|
122.24 ± 4.24 b
|
24
|
104.00 ± 3.50d
|
132.00 ± 11.4c
|
157.00 ± 2.80a
|
116.00 ± 3.70b
|
113.92 ± 5.80b
|
48
|
103.00 ± 3.27a
|
102.00 ± 4.87a
|
104.00 ± 3.89a
|
99.00 ± 2.91a
|
104.00 ± 5.17a
|
Liver cells
|
CP
|
12
|
141.67 ± 0.08b
|
163.33 ± 0.6a
|
130.00 ± 0.33b
|
110.00 ± 2.60c
|
103.33 ± 1.70c
|
24
|
127.50 ± 0.10a
|
102.50 ± 1.25c
|
87.50 ± 1.20e
|
95.00 ± 1.60 d
|
112.50 ± 1.13b
|
48
|
68.09 ± 0.40b
|
59.57 ± 1.50c
|
59.57 ± 2.67c
|
78.72 ± 0.88a
|
80.85 ± 1.29 a
|
CPS
|
12
|
99.87 ± 1.89b
|
99.76 ± 0.88b
|
102.41 ± 1.57b
|
115.84 ± 3.74a
|
114.07 ± 0.72a
|
24
|
100.10 ± 1.26b
|
102.34 ± 1.26b
|
101.79 ± 2.19b
|
117.25 ± 1.99a
|
114.96 ± 6.46a
|
48
|
99.12 ± 0.76b
|
97.37 ± 1.89b
|
96.01 ± 2.93b
|
102.09 ± 0.94a
|
102.94 ± 2.24a
|
Kidney cells
|
CP
|
12
|
124.30 ± 4.67b
|
138.32 ± 1.87a
|
106.54 ± 1.87c
|
123.36 ± 10.28b
|
118.69 ± 4.67b
|
24
|
120.00 ± 7.62 B
|
130.05 ± 2.86a
|
72.38 ± 21.90c
|
53.33 ± 4.76d
|
56.19 ± 6.67d
|
48
|
67.24 ± 3.45a
|
51.72 ± 1.72b
|
54.31 ± 19.83b
|
29.31 ± 8.62c
|
33.62 ± 4.31c
|
CPS
|
12
|
101.37 ± 1.18b
|
99.50 ± 2.26b
|
105.29 ± 1.34a
|
97.56 ± 3.67b
|
89.25 ± 1.28c
|
24
|
100.58 ± 2.12b
|
102.25 ± 2.14b
|
111.30 ± 0.94a
|
100.41 ± 2.97b
|
77.78 ± 2.07c
|
48
|
100.28 ± 1.33 B
|
103.65 ± 2.43b
|
106.72 ± 5.81a
|
90.00 ± 2.05c
|
48.28 ± 2.66d
|
- Data were expressed as mean ± SD (n = 8). The different lowercase letters in the same row indicate significant difference from each other (p < 0.05), while the same lowercase letters in the same row indicate insignificant difference from each other (p > 0.05). CP: compound probiotics (for intestinal epithelium cell incubation); CPS: cell-free compound probiotics supernatant (for liver and kidney cell incubation). The final counts of B. subtilis, L. casein, E. faecalis and C. utilis in CP were designed as 1.0 × 102, 1.0 × 102, 1.0 × 104 and 1.0 × 102 CFU/mL to make CP1; 1.0 × 103, 1.0 × 103, 1.0 × 105 and 1.0 × 103 CFU/mL to make CP2; 1.0 × 104, 1.0 × 104, 1.0 × 106 and 1.0 × 104 CFU/mL to make CP3; 1.0 × 105, 1.0 × 105, 1.0 × 107 and 1.0 × 105 CFU/mL to make CP4; 1.0 × 106, 1.0 × 106, 1.0 × 108 and 1.0 × 106 CFU/mL to make CP5, respectively. Their corresponding supernatants were combined together to make CPS1, CPS2, CPS3, CPS4 and CPS5