Effects of compound probiotics and aflatoxin-degradation enzyme on alleviating aflatoxin-induced cytotoxicity in chicken embryo primary intestinal epithelium, liver and kidney cells

Guo, Hong-Wei; Chang, Juan; Wang, Ping; Yin, Qing-Qiang; Liu, Chao-Qi; Xu, Xiao-Xiang; Dang, Xiao-Wei; Hu, Xiao-Fei; Wang, Quan-Liang

doi:10.1186/s13568-021-01196-7

AMB Express

Table 4 Effects of different CP or CPS concentrations and incubation time on primary cell viabilities (%)

From: Effects of compound probiotics and aflatoxin-degradation enzyme on alleviating aflatoxin-induced cytotoxicity in chicken embryo primary intestinal epithelium, liver and kidney cells

	Time (h)	CP1 or CPS1	CP2 or CPS2	CP3 or CPS3	CP4 or CPS4	CP5 or CPS5
Intestinal epithelium cells
CP	12	198.25 ± 10.53^b	231.58 ± 5.26^c	157.89 ± 5.26^a	145.61 ± 7.02^a	207.02 ± 3.51^b
	24	130.23 ± 9.30^a	120.93 ± 11.16^ab	90.70 ± 8.10^d	109.3 ± 7.67^c	109.30 ± 6.60^bc
	48	84.78 ± 8.70^a	80.43 ± 4.35^a	60.87 ± 3.04^b	47.83 ± 4.35^c	50.00 ± 3.52^c
CPS	12	116.85 ± 5.01^bc	113.88 ± 4.87^c	105.39 ± 1.52^d	136.13 ± 1.59 ^a	122.24 ± 4.24 ^b
	24	104.00 ± 3.50^d	132.00 ± 11.4^c	157.00 ± 2.80^a	116.00 ± 3.70^b	113.92 ± 5.80^b
	48	103.00 ± 3.27^a	102.00 ± 4.87^a	104.00 ± 3.89^a	99.00 ± 2.91^a	104.00 ± 5.17^a
Liver cells
CP	12	141.67 ± 0.08^b	163.33 ± 0.6^a	130.00 ± 0.33^b	110.00 ± 2.60^c	103.33 ± 1.70^c
	24	127.50 ± 0.10^a	102.50 ± 1.25^c	87.50 ± 1.20^e	95.00 ± 1.60 ^d	112.50 ± 1.13^b
	48	68.09 ± 0.40^b	59.57 ± 1.50^c	59.57 ± 2.67^c	78.72 ± 0.88^a	80.85 ± 1.29 ^a
CPS	12	99.87 ± 1.89^b	99.76 ± 0.88^b	102.41 ± 1.57^b	115.84 ± 3.74^a	114.07 ± 0.72^a
	24	100.10 ± 1.26^b	102.34 ± 1.26^b	101.79 ± 2.19^b	117.25 ± 1.99^a	114.96 ± 6.46^a
	48	99.12 ± 0.76^b	97.37 ± 1.89^b	96.01 ± 2.93^b	102.09 ± 0.94^a	102.94 ± 2.24^a
Kidney cells
CP	12	124.30 ± 4.67^b	138.32 ± 1.87^a	106.54 ± 1.87^c	123.36 ± 10.28^b	118.69 ± 4.67^b
	24	120.00 ± 7.62 ^B	130.05 ± 2.86^a	72.38 ± 21.90^c	53.33 ± 4.76^d	56.19 ± 6.67^d
	48	67.24 ± 3.45^a	51.72 ± 1.72^b	54.31 ± 19.83^b	29.31 ± 8.62^c	33.62 ± 4.31^c
CPS	12	101.37 ± 1.18^b	99.50 ± 2.26^b	105.29 ± 1.34^a	97.56 ± 3.67^b	89.25 ± 1.28^c
	24	100.58 ± 2.12^b	102.25 ± 2.14^b	111.30 ± 0.94^a	100.41 ± 2.97^b	77.78 ± 2.07^c
	48	100.28 ± 1.33 ^B	103.65 ± 2.43^b	106.72 ± 5.81^a	90.00 ± 2.05^c	48.28 ± 2.66^d

Data were expressed as mean ± SD (n = 8). The different lowercase letters in the same row indicate significant difference from each other (p < 0.05), while the same lowercase letters in the same row indicate insignificant difference from each other (p > 0.05). CP: compound probiotics (for intestinal epithelium cell incubation); CPS: cell-free compound probiotics supernatant (for liver and kidney cell incubation). The final counts of B. subtilis, L. casein, E. faecalis and C. utilis in CP were designed as 1.0 × 10², 1.0 × 10², 1.0 × 10⁴ and 1.0 × 10² CFU/mL to make CP1; 1.0 × 10³, 1.0 × 10³, 1.0 × 10⁵ and 1.0 × 10³ CFU/mL to make CP2; 1.0 × 10⁴, 1.0 × 10⁴, 1.0 × 10⁶ and 1.0 × 10⁴ CFU/mL to make CP3; 1.0 × 10⁵, 1.0 × 10⁵, 1.0 × 10⁷ and 1.0 × 10⁵ CFU/mL to make CP4; 1.0 × 10⁶, 1.0 × 10⁶, 1.0 × 10⁸ and 1.0 × 10⁶ CFU/mL to make CP5, respectively. Their corresponding supernatants were combined together to make CPS1, CPS2, CPS3, CPS4 and CPS5

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