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Table 4 Effects of different CP or CPS concentrations and incubation time on primary cell viabilities (%)

From: Effects of compound probiotics and aflatoxin-degradation enzyme on alleviating aflatoxin-induced cytotoxicity in chicken embryo primary intestinal epithelium, liver and kidney cells

  Time (h) CP1 or CPS1 CP2 or CPS2 CP3 or CPS3 CP4 or CPS4 CP5 or CPS5
Intestinal epithelium cells
 CP 12 198.25 ± 10.53b 231.58 ± 5.26c 157.89 ± 5.26a 145.61 ± 7.02a 207.02 ± 3.51b
24 130.23 ± 9.30a 120.93 ± 11.16ab 90.70 ± 8.10d 109.3 ± 7.67c 109.30 ± 6.60bc
48 84.78 ± 8.70a 80.43 ± 4.35a 60.87 ± 3.04b 47.83 ± 4.35c 50.00 ± 3.52c
 CPS 12 116.85 ± 5.01bc 113.88 ± 4.87c 105.39 ± 1.52d 136.13 ± 1.59 a 122.24 ± 4.24 b
24 104.00 ± 3.50d 132.00 ± 11.4c 157.00 ± 2.80a 116.00 ± 3.70b 113.92 ± 5.80b
48 103.00 ± 3.27a 102.00 ± 4.87a 104.00 ± 3.89a 99.00 ± 2.91a 104.00 ± 5.17a
Liver cells
 CP 12 141.67 ± 0.08b 163.33 ± 0.6a 130.00 ± 0.33b 110.00 ± 2.60c 103.33 ± 1.70c
24 127.50 ± 0.10a 102.50 ± 1.25c 87.50 ± 1.20e 95.00 ± 1.60 d 112.50 ± 1.13b
48 68.09 ± 0.40b 59.57 ± 1.50c 59.57 ± 2.67c 78.72 ± 0.88a 80.85 ± 1.29 a
 CPS 12 99.87 ± 1.89b 99.76 ± 0.88b 102.41 ± 1.57b 115.84 ± 3.74a 114.07 ± 0.72a
24 100.10 ± 1.26b 102.34 ± 1.26b 101.79 ± 2.19b 117.25 ± 1.99a 114.96 ± 6.46a
48 99.12 ± 0.76b 97.37 ± 1.89b 96.01 ± 2.93b 102.09 ± 0.94a 102.94 ± 2.24a
Kidney cells
 CP 12 124.30 ± 4.67b 138.32 ± 1.87a 106.54 ± 1.87c 123.36 ± 10.28b 118.69 ± 4.67b
24 120.00 ± 7.62 B 130.05 ± 2.86a 72.38 ± 21.90c 53.33 ± 4.76d 56.19 ± 6.67d
48 67.24 ± 3.45a 51.72 ± 1.72b 54.31 ± 19.83b 29.31 ± 8.62c 33.62 ± 4.31c
 CPS 12 101.37 ± 1.18b 99.50 ± 2.26b 105.29 ± 1.34a 97.56 ± 3.67b 89.25 ± 1.28c
24 100.58 ± 2.12b 102.25 ± 2.14b 111.30 ± 0.94a 100.41 ± 2.97b 77.78 ± 2.07c
48 100.28 ± 1.33 B 103.65 ± 2.43b 106.72 ± 5.81a 90.00 ± 2.05c 48.28 ± 2.66d
  1. Data were expressed as mean ± SD (n = 8). The different lowercase letters in the same row indicate significant difference from each other (p < 0.05), while the same lowercase letters in the same row indicate insignificant difference from each other (p > 0.05). CP: compound probiotics (for intestinal epithelium cell incubation); CPS: cell-free compound probiotics supernatant (for liver and kidney cell incubation). The final counts of B. subtilis, L. casein, E. faecalis and C. utilis in CP were designed as 1.0 × 102, 1.0 × 102, 1.0 × 104 and 1.0 × 102 CFU/mL to make CP1; 1.0 × 103, 1.0 × 103, 1.0 × 105 and 1.0 × 103 CFU/mL to make CP2; 1.0 × 104, 1.0 × 104, 1.0 × 106 and 1.0 × 104 CFU/mL to make CP3; 1.0 × 105, 1.0 × 105, 1.0 × 107 and 1.0 × 105 CFU/mL to make CP4; 1.0 × 106, 1.0 × 106, 1.0 × 108 and 1.0 × 106 CFU/mL to make CP5, respectively. Their corresponding supernatants were combined together to make CPS1, CPS2, CPS3, CPS4 and CPS5