Table 2 Primer sequences of some genes for quantitative RT-PCR
Gene | Accession number | Primer sequence (5′–3′) |
|---|---|---|
β-actin | LO8165 | F: GAGAAATTGTGCGTGACATCA |
R: CCTGAACCTCTCATTGCCA | ||
IL-6 | AJ309540 | F: CAAGGTGACGGAGGAGGAC |
R: TGGCGAGGAGGGATTTCT | ||
IL-8 | AJ009800 | F: ATGAACGGCAAGCTTGGAGCTG |
R: TCCAAGCACACCTCTCTTCCATCC | ||
iNOS | U46504 | F: CAGCTGATTGGGTGTGGAT |
R: TTTCTTTGGCCTACGGGTC | ||
NF-κBp65 | NM_205129 | F: GTGTGAAGAAACGGGAACTG |
R: GGCACGGTTGTCATAGATGG | ||
TNF-α | NM_204267 | F: GAGCGTTGACTTGGCTGTC |
R: AAGCAACAACCAGCTATGCAC | ||
NOD1 | JX465487 | F: AGCACTGTCCATCCTCTGTCC |
R: TGAGGGTTGGTAAAGGTCTGCT | ||
TLR2 | NM_001161650 | F: GGGGCTCAGGCAAAATC |
R: AGCAGGGTTCTCAGGTTCACA |