Skip to main content

Table 1 Primer sequences for site-directed mutagenesis

From: Engineering a lipoxygenase from cyclocybe aegerita towards long chain polyunsaturated fatty acids

primer

sequence

fw_Lox1-I393F

5′-ccgagtatggttgccccgtttagttattttaaaattccgg-3′

rv_Lox1-I393F

5′-ccggaattttaaaataactaaacggggcaaccatactcgg-3′

fw_Lox1-V581A

5′-gatgatggcacaggcgccgtatctgctgag-3′

rv_Lox1-V581A

5′-ctcagcagatacggcgcctgtgccatcatc-3′

fw_Lox1-V581F

5′-gatgatggcacagtttccgtatctgctgag-3′′

rv_Lox1-V581F

5′-ctcagcagatacggaaactgtgccatcatc-3′

fw_Lox1-W330L

5′-caggtgagcgatctgacccgtcatgaactg-3′

rv_Lox1-W330L

5′-cagttcatgacgggtcagatcgctcacctg-3′