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Table 1 Primer sequences for site-directed mutagenesis

From: Engineering a lipoxygenase from cyclocybe aegerita towards long chain polyunsaturated fatty acids

primer sequence
fw_Lox1-I393F 5′-ccgagtatggttgccccgtttagttattttaaaattccgg-3′
rv_Lox1-I393F 5′-ccggaattttaaaataactaaacggggcaaccatactcgg-3′
fw_Lox1-V581A 5′-gatgatggcacaggcgccgtatctgctgag-3′
rv_Lox1-V581A 5′-ctcagcagatacggcgcctgtgccatcatc-3′
fw_Lox1-V581F 5′-gatgatggcacagtttccgtatctgctgag-3′′
rv_Lox1-V581F 5′-ctcagcagatacggaaactgtgccatcatc-3′
fw_Lox1-W330L 5′-caggtgagcgatctgacccgtcatgaactg-3′
rv_Lox1-W330L 5′-cagttcatgacgggtcagatcgctcacctg-3′