Fig. 1

a Partial sequence alignment of various LOX. Cyclocybe aegerita CaeLox1 (MW013781), CaeLox4 (MK451709), Pleurotus sapidus PsaLOX (CCV01580), Pleurotus ostreatus PosLOX (CCV01578). Alignment was carried out by using Clustal Omega with default parameters. The highlighted amino acid residues involved in iron binding are highlighted in blue. Amino acids considered to be involved in selectivity and activity by shaping the substrate tunnel are highlighted in red. Amino acid residues considered to interact with the carboxylate end of the substrate via ionic interactions are highlighted in yellow. b Substrate tunnel of the homology model of CaeLox1. Iron (orange) binding amino acid residues are highlighted as blue sticks (H338, H343, H523, N527, I656) and the positively charged amino acid at the bottom of the substrate tunnel is highlighted in yellow sticks. Amino acid residues involved in shaping the substrate tunnel are shown as red sticks (V581, I393 and W330). Oxygen inside the amino acid side chains are colored in red and nitrogen in blue