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Fig. 1 | AMB Express

Fig. 1

From: Efficient genome editing with CRISPR/Cas9 in Pleurotus ostreatus

Fig. 1

Targeted gene replacement using homologous recombination along with CRISPR/Cas9. a A schematic diagram of the procedure used to construct the donor DNA template that lacks the sgRNA sequence for homologous recombination. b A schematic diagram showing the method for fcy1 mutation using CRISPR/Cas9 and donor DNA. PCR fragments with around 1 kb, 0.5 kb, and 0.2 kb each of right and left homologous arms were used as donor DNA temples. Black arrows display the primer pairs used for the PCR experiments in Additional file 1: Fig. S2C

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