Fig. 1From: Efficient genome editing with CRISPR/Cas9 in Pleurotus ostreatusTargeted gene replacement using homologous recombination along with CRISPR/Cas9. a A schematic diagram of the procedure used to construct the donor DNA template that lacks the sgRNA sequence for homologous recombination. b A schematic diagram showing the method for fcy1 mutation using CRISPR/Cas9 and donor DNA. PCR fragments with around 1Â kb, 0.5Â kb, and 0.2Â kb each of right and left homologous arms were used as donor DNA temples. Black arrows display the primer pairs used for the PCR experiments in Additional file 1: Fig. S2CBack to article page