Table 1 Randomized central composite design of response surface methodology for evaluating interactions between some important factors influencing enzyme(s) production from A. niger APS under surface fermentation
Run | Block | CSL % (A) | CaSO4% (B) | MgSO4% (C) | Tween 80% (D) |
|---|---|---|---|---|---|
1 | Block 1 | − 1 | − 1 | + 1 | − 1 |
2 | Block 1 | + 1 | + 1 | − 1 | + 1 |
3 | Block 1 | − 1 | + 1 | − 1 | − 1 |
4 | Block 1 | 0 | 0 | 0 | 0 |
5 | Block 1 | + 1 | − 1 | − 1 | − 1 |
6 | Block 1 | 0 | 0 | 0 | 0 |
7 | Block 1 | − 1 | + 1 | + 1 | + 1 |
8 | Block 1 | + 1 | + 1 | + 1 | − 1 |
9 | Block 1 | − 1 | − 1 | − 1 | + 1 |
10 | Block 1 | + 1 | − 1 | + 1 | + 1 |
11 | Block 2 | − 1 | − 1 | + 1 | + 1 |
12 | Block 2 | + 1 | − 1 | + 1 | − 1 |
13 | Block 2 | + 1 | + 1 | − 1 | − 1 |
14 | Block 2 | 0 | 0 | 0 | 0 |
15 | Block 2 | − 1 | + 1 | + 1 | − 1 |
16 | Block 2 | − 1 | − 1 | − 1 | − 1 |
17 | Block 2 | + 1 | + 1 | + 1 | + 1 |
18 | Block 2 | − 1 | + 1 | − 1 | + 1 |
19 | Block 2 | 0 | 0 | 0 | 0 |
20 | Block 2 | + 1 | − 1 | − 1 | + 1 |
21 | Block 3 | 0 | 0 | 0 | 0 |
22 | Block 3 | 0 | 0 | 0 | − 2 |
23 | Block 3 | 0 | 0 | + 2 | 0 |
24 | Block 3 | 0 | 0 | − 2 | 0 |
25 | Block 3 | 0 | 0 | 0 | 0 |
26 | Block 3 | 0 | + 2 | 0 | 0 |
27 | Block 3 | + 2 | 0 | 0 | 0 |
28 | Block 3 | 0 | 0 | 0 | + 2 |
29 | Block 3 | 0 | − 2 | 0 | 0 |
30 | Block 3 | − 2 | 0 | 0 | 0 |