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Table 1 Randomized central composite design of response surface methodology for evaluating interactions between some important factors influencing enzyme(s) production from A. niger APS under surface fermentation

From: Cocktail of carbohydrases from Aspergillus niger: an economical and eco-friendly option for biofilm clearance from biopolymer surfaces

Run Block CSL % (A) CaSO4% (B) MgSO4% (C) Tween 80% (D)
1 Block 1 − 1 − 1 + 1 − 1
2 Block 1 + 1 + 1 − 1 + 1
3 Block 1 − 1 + 1 − 1 − 1
4 Block 1 0 0 0 0
5 Block 1 + 1 − 1 − 1 − 1
6 Block 1 0 0 0 0
7 Block 1 − 1 + 1 + 1 + 1
8 Block 1 + 1 + 1 + 1 − 1
9 Block 1 − 1 − 1 − 1 + 1
10 Block 1 + 1 − 1 + 1 + 1
11 Block 2 − 1 − 1 + 1 + 1
12 Block 2 + 1 − 1 + 1 − 1
13 Block 2 + 1 + 1 − 1 − 1
14 Block 2 0 0 0 0
15 Block 2 − 1 + 1 + 1 − 1
16 Block 2 − 1 − 1 − 1 − 1
17 Block 2 + 1 + 1 + 1 + 1
18 Block 2 − 1 + 1 − 1 + 1
19 Block 2 0 0 0 0
20 Block 2 + 1 − 1 − 1 + 1
21 Block 3 0 0 0 0
22 Block 3 0 0 0 − 2
23 Block 3 0 0 + 2 0
24 Block 3 0 0 − 2 0
25 Block 3 0 0 0 0
26 Block 3 0 + 2 0 0
27 Block 3 + 2 0 0 0
28 Block 3 0 0 0 + 2
29 Block 3 0 − 2 0 0
30 Block 3 − 2 0 0 0
  1. For CSL; − 2 = 0.4%, − 1 = 0.6%, 0 = 0.8%, + 1 = 1.0%, + 2 = 1.2%; CaSO4; − 2 = 0.01%, − 1 = 0.015%, 0 = 0.02%, + 1 = 0.025, + 2 = 0.03%; MgSO4; − 2 = 0.01%, − 1 = 0.015%, 0 = 0.02%, + 1 = 0.025, + 2 = 0.03%; Tween 80; − 2 = 0.02%, − 1 = 0.04%, 0 = 0.06%, + 1 = 0.08%, + 2 = 0.1%
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