Fig. 6
Effects of QT, PGEA, CPFLM, and the nanoconjugates of natural compounds mentioned above on the excystation inhibition of A. castellanii cysts. 1 × 105 cysts were incubated with 5 and 10 μM (or mg/mL) of natural compounds or nanoconjugates for 72 h at 30 °C then the viability of A. castellanii trophozoites were determined by staining with 0.1% Trypan blue. The nanoconjugates of quercetin (10 μM) showed significantly increased results as compared to AgNPs and QT alone (#P < 0.05, using two samples T-test, two-tailed distribution). PGEA and CPFLM alone showed significant inhibition of A. castellanii excystation when compared to the negative control (cysts alone) (*P < 0.05, using two samples T-test, two-tailed distribution)