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Fig. 5 | AMB Express

Fig. 5

From: Antiamoebic activity of plant-based natural products and their conjugated silver nanoparticles against Acanthamoeba castellanii (ATCC 50492)

Fig. 5

Effects of QT, PGEA, CPFLM and the nanoconjugates of the natural compounds above mentioned on the encystation of A. castellanii. 5 × 105 amoebae were incubated with encystation media (E.M, including 10% glucose and 5 μM MgCl2) and respective 5 and 10 μM of compounds or nanoconjugates for 72 h at 30 °C. Then the cysts were counted after adding 0.05% of Sodium dodecyl sulfate (SDS). 10 μM of QT-AgNPs showed significantly enhanced encystation activity as compared to QT and AgNPs alone (#P < 0.05, using two sample T-test, two-tailed distribution). PGEA and CPFLM alone showed significant inhibition of A. castellanii encystation when compared to the negative control (amoeba + E.M) (*P < 0.05, using two sample T-test, two-tailed distribution)

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