Fig. 3
Amoebicidal effects of QT, PGEA, CPFLM and the nanoconjugates of the natural compounds above mentioned against A. castellanii. 5 × 105A. castellanii was incubated with 5 and 10 μM (or mg/mL) of the natural compounds and the nanoconjugates for 24 h at 30 °C then the viability of A. castellanii was determined by staining with 0.1% Trypan Blue. The natural products QT, PGEA and CPFLM showed significant inhibition of A. castellanii growth when compared to the negative control (amoeba alone) (*P < 0.05, using two sample T-test, two-tailed distribution). Nanoparticles conjugation enhanced the antimaoebic activity of natural products as compared to AgNPs and compounds alone (*P < 0.05, using two sample T-test, two-tailed distribution). #shows significance as compared to compound and AgNPs alone