Bactericidal activity of Myrrh extracts and two dosage forms against standard bacterial strains and multidrug-resistant clinical isolates with GC/MS profiling

AMB Express

Table 7 Identified constituents in hexane extract and essential oil of Myrrh

No.	Compound	KI*	Rel. abundance (%)		Methods of identification
No.	Compound	KI*	Hexane extract	Essential oil	Methods of identification
Oxygenated monoterpenes
1	Eugenol	1532	3.15 ± 0.32	–	KI, MS, AT
2	Cuminaldehyde	1478	0.71 ± 0.05	–	KI, MS, AT
			3.86
Sesquiterpene hydrocarbons
3	γ-elemene	1420	0.95 ± 0.12	–	KI, MS, AT
4	δ-Cadinene	1460	4.29 ± 0.21^a	4.20 ± 0.32^a	KI, MS, AT
			5.24	4.20
Oxygenated sesquiterpenes
5	α-copaene-8-ol	1383	1.42 ± 0.12^a	0.47 ± 0.09^b	KI, MS, AT
6	Furanodiene	1498	10.29 ± 0.75^a	4.60 ± 0.24^b	KI, MS
7	Furanoeudesma-1,3-diene	1518	15.99 ± 0.88^a	20.40 ± 2.01^b	KI, MS
8	Lindestrene	1520	5.69 ± 0.95^a	3.60 ± 0.85^a	KI, MS
9	Isofuranogermacrene	1452	14.26 ± 0.72^a	13.52 ± 1.62^a	KI, MS
10	2-methoxyfuranodiene	1548	3.87 ± 0.33	–	KI, MS
11	2-methoxyfurano-guaia-9-en-8-ne	1572	3.15 ± 0.74	–	KI, MS
12	2-acetoxy-furano-diene	1605	9.21 ± 1.23^a	26.82 ± 2.08^b	KI, MS
13	Guaiol	1455	1.67 ± 0.78^a	8.79 ± 0.68^b	KI, MS
14	Epi-curzerenone	1510	0.56 ± 0.06	–	KI, MS, AT
15	Furanoeudesma-1,4-dien-6-one	1518	7.96 ± 0.46^a	17.59 ± 2.79^b	KI, MS
16	Furanodiene-6-one	1620	6.12 ± 1.66	–	KI, MS
17	4,5-dihydrofuranodiene-6-one	1560	2.72 ± 0.29	–	KI, MS
			82.91	95.79
	Total % of identified compounds		92.01	99.99
	Total number of identified compounds		17	9

MS: Identification based on mass spectral data; AT: Identification based on co-chromatography with authentic samples, (–) = not detected
Values are ± SEM (n = 3), means followed by different letters in same row denote significant difference at p < 0.05, paired-t-test
* KI: Kovat’s index calculated using homologous series of n-alkanes