Table 4 CRISPR-PCDup can duplicate up to 400kb of chromosomal region
Size of the duplication (Name of region) | gRNA | No. of transformants | Proportion of correct transformants |
|---|---|---|---|
400 kb (Chr15-L1) | + | 25 | 20% (2/10) |
– | 0 | – | |
350 kb (Chr15-L2) | + | 40 | 20% (2/10) |
– | 0 | – | |
300 kb (Chr15-L3) | + | 733 | 3% (1/35) |
– | 0 | – | |
250 kb (Chr15-L4) | + | 114 | 20% (2/10) |
– | 0 | – | |
200 kb (Chr15-L5) | + | 120 | 90% (9/10) |
– | 0 | – |