Table 3 CRISPR-PCDup increases duplication efficiency and induces simultaneous double duplication
Duplicated regions | Method | Number of transformants (n) | Karyotype analysis (n) | Correct transformants (n) |
|---|---|---|---|---|
Chr3-1 (158 kb) | CRISPR-PCDup | 62 | 8 | 8 (100%) |
Conventional PCDup | 2 | 2 | 0 (0%) | |
Chr3-2 (160 kb) | CRISPR-PCDup | 1316 | 8 | 8 (100%) |
Conventional PCDup | 51 | 4 | 0 (0%) | |
Chr3-2 (160 kb) and Chr15-L5 (200 kb) | CRISPR-PCDup | 75 | 25 | 10 (40%) |
Conventional PCDup | 0 | – | – |