Table 1 Strains and plasmids used in the CRISPR-PCDup experiments
Strain or plasmid | Description | Remarks |
|---|---|---|
Strain | Â | Â |
FY833 | MATa ura3-52 his3-Δ200 leu2Δ1 lys2Δ202 trp1Δ63 | (Winston et al. 1995) |
SJY415 | Trp+ transformants of FY833 harboring plasmid p414-TEF1p-Cas9-CYC1t | This study |
FY834 | MATα ura3-52 his3-Δ200 leu2Δ1 lys2Δ202 trp1Δ63 | (Winston et al. 1995) |
SJY30 | Trp+ transformants of FY834 harboring plasmid p414-TEF1p-Cas9-CYC1t | (Sasano et al. 2016) |
Plasmid | Â | Â |
pUG6 | Containing loxP-flanked marker gene deletion cassette: loxP-pAgTEF1-kanMX-tAgTEF1-loxP | (Güldener et al. 1996) |
p3009 | The loxP-CgHIS3-loxP module containing plasmid constructed by modifying pUG6 | (Sugiyama et al. 2005) |
p3121 | The CEN4 module containing plasmid constructed by modifying pUG6 | (Sugiyama et al. 2005) |
p3122 | The CEN4-loxP-CgLEU2-loxP module containing plasmid constructed by modifying pUG6 | (Sugiyama et al. 2008) |
p3123 | The CEN4-loxP-CgHIS3-loxP module containing plasmid constructed by modifying pUG6 | (Sugiyama et al. 2008) |
pSJ23 | A derivative of pUG6 carrying URA3 | (Easmin et al. 2019b) |
pSJ69 | loxP site-deleted p3008 | (Easmin et al. 2019a) |
pSJ70 | loxP site deleted p3009 | (Easmin et al. 2019b) |
p414-TEF1p-Cas9- CYC1t | TEF1p-Cas9-CYC1t module containing YCp type plasmid | (DiCarlo et al. 2013) |
p426-SNR52p-gRNA.CAN1.Y-SUP4t | SNR52p-gRNA.CAN1 Y-SUP4t module containing YEp type plasmid | (DiCarlo et al. 2013) |