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Table 1 Strains and plasmids used in the CRISPR-PCDup experiments

From: CRISPR-PCDup: a novel approach for simultaneous segmental chromosomal duplication in Saccharomyces cerevisiae

Strain or plasmidDescriptionRemarks
Strain  
FY833MATa ura3-52 his3-Δ200 leu2Δ1 lys2Δ202 trp1Δ63(Winston et al. 1995)
SJY415Trp+ transformants of FY833 harboring plasmid p414-TEF1p-Cas9-CYC1tThis study
FY834MATα ura3-52 his3-Δ200 leu2Δ1 lys2Δ202 trp1Δ63(Winston et al. 1995)
SJY30Trp+ transformants of FY834 harboring plasmid p414-TEF1p-Cas9-CYC1t(Sasano et al. 2016)
Plasmid  
pUG6Containing loxP-flanked marker gene deletion cassette: loxP-pAgTEF1-kanMX-tAgTEF1-loxP(Güldener et al. 1996)
p3009The loxP-CgHIS3-loxP module containing plasmid constructed by modifying pUG6(Sugiyama et al. 2005)
p3121The CEN4 module containing plasmid constructed by modifying pUG6(Sugiyama et al. 2005)
p3122The CEN4-loxP-CgLEU2-loxP module containing plasmid constructed by modifying pUG6(Sugiyama et al. 2008)
p3123The CEN4-loxP-CgHIS3-loxP module containing plasmid constructed by modifying pUG6(Sugiyama et al. 2008)
pSJ23A derivative of pUG6 carrying URA3(Easmin et al. 2019b)
pSJ69loxP site-deleted p3008(Easmin et al. 2019a)
pSJ70loxP site deleted p3009(Easmin et al. 2019b)
p414-TEF1p-Cas9- CYC1tTEF1p-Cas9-CYC1t module containing YCp type plasmid(DiCarlo et al. 2013)
p426-SNR52p-gRNA.CAN1.Y-SUP4tSNR52p-gRNA.CAN1 Y-SUP4t module containing YEp type plasmid(DiCarlo et al. 2013)