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Fig. 3 | AMB Express

Fig. 3

From: CRISPR-PCDup: a novel approach for simultaneous segmental chromosomal duplication in Saccharomyces cerevisiae

Fig. 3

Simultaneous double duplication in the Chr3-2 and Chr15-L5 regions. The Chr3-2 region of Chr3 and the Chr15-L5 region of Chr15 were simultaneously duplicated by CRISPR-PCDup. The duplicating module of Chr3-2 was marked with CEN4 and CgLEU2; C15-L5 was marked with CEN4 and CgHIS3. After duplication, 2 derived chromosomes are expected to be generated; 160 kb from the C3-2 region and 200 kb from the C15-L5 region. Fig. 3a represents PFGE analysis of the wild-type strain, SJY30 and 10 randomly chosen transformants. Fig 3b, c shows the results of Southern blot analysis for detecting Chr15 (1091 kb) and the newly duplicated 200 kb chromosome along with Chr3 (317 kb) and the newly duplicated 160 kb chromosome, respectively

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