Fig. 7

The evaluation of the oral anti-porcine epidemic diarrhea (PEDV) spike (S)-specific IgA in piglets by the ELISA. Orally vaccinations were administered in 2-week intervals on week 0 (priming), week 2 (first boosting), and week 4 (second boosting). The blood samples were collected on week 0, week 2, week 4, and week 6. The data is shown as the means of optical density (OD) values with standard deviation (SD, the error bars). The solid line with round or square icons represented the trend of IgG level of the S-Bm pupae group or S-Bm cell group, respectively. The dotted line with triangle or diamond icons represented the trend of IgG level of the WT-Bm pupae or Medium-control groups. No significant difference with the control group (p < 0.05) were observed