Fig. 5

Detection of porcine epidemic diarrhea virus spike (PEDV S) protein expression in the S-Bm inoculated pupae. a The images of the pupae 3 days after the S-Bm inoculation under the visible light (left) or the UV emission (right). The EGFP signal of the S-Bm inoculated pupae was detected. Control: the pupae without S-Bm inoculation; S-Bm: the S-Bm inoculated pupae. b The distribution of the EGFP-conjugated recombinant S protein on the pupae. c The evaluation of recombinant S protein expression level of the pupae by the western blotting. The proteins from the homogenized body of the pupae were lysed, denatured, separated by protein electrophoresis, transferred onto the PVDF membrane, and probed with the anti-6× His tag antibody. The corresponding size of the S protein was predicted about 170–200 kDa. Single star icon represented the intact S protein on the predicted molecular weight; the double star icon represented the possible molecular weight of the cleaved S protein