Fig. 4From: Oral administration of porcine epidemic diarrhea virus spike protein expressing in silkworm pupae failed to elicit immune responses in pigsDetection of porcine epidemic diarrhea virus spike (PEDV S) protein expression level in the cell lysate of S-Bm inoculated BmN cells. The BmN cells inoculated with 10 multiplicity of infection (MOI) of the S-Bm were harvested and lysed 3 days after the inoculation. The proteins in the cellular lysate were separated by protein electrophoresis, transferred onto the PVDF membrane, and probed with the anti-6× His tag antibody. a The characterization of PEDV S protein from S-Bm inoculated BmN cells. Cell only: the samples from non-inoculated BmN cells; wild-type: the samples from wild type BmNPV inoculated BmN cells; S-Bm the samples from the S-Bm inoculated BmN cells. Single star icon represented the intact S protein on the predicted molecular weight; the double star icon represented the possible molecular weight of the cleaved S protein. b The determination of the yield of the PEDV S protein from S-Bm inoculated BmN cells. S-Bm the samples from the S-Bm inoculated BmN cells, standard the standard proteins of 6× His, GAPDH control cellular proteinBack to article page