Fig. 4From: Enhancement of pullulanase production from recombinant Bacillus subtilis by optimization of feeding strategy and fermentation conditionsEffect of feeding concentration on OD600 (a) and enzyme activity (b). Glucose was fed at 300 g L−1 (black curve), 400 g L−1 (orange curve), and 500 g L−1 (blue curve), respectively. The averages of three independent experiments together with the corresponding standard deviations are shown for all values of OD600 and enzyme activityBack to article page