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AMB Express

Table 4 Analysis of LbADHWT and LbADHK71E activity for the substrates methyl acetoacetate, 2-acetylpyridine, 4-hydroxy-2-butanone, acetophenone, and 2-hexanone

From: NADPH biosensor-based identification of an alcohol dehydrogenase variant with improved catalytic properties caused by a single charge reversal at the protein surface

Substrate

Substrate concentration (mM)

LbADHWT activity (µmol min−1 mg−1)

LbADHK71E activity (µmol min−1 mg−1)

% increase

2-Acetylpyridine

10

104.1 ± 1.7

125.5 ± 1.1

21

2-Hexanone

10

22.8 ± 0.7

27.9 ± 1.1

22

acetophenone

5

29.8 ± 0.7

39.5 ± 0.7

33

4-Hydroxy-2-butanone

10

39.5 ± 1.3

54.9 ± 4.6

39

  1. Mean values obtained from a single protein preparation and three technical replicates are shown
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