Skip to main content

Table 4 Analysis of LbADHWT and LbADHK71E activity for the substrates methyl acetoacetate, 2-acetylpyridine, 4-hydroxy-2-butanone, acetophenone, and 2-hexanone

From: NADPH biosensor-based identification of an alcohol dehydrogenase variant with improved catalytic properties caused by a single charge reversal at the protein surface

Substrate Substrate concentration (mM) LbADHWT activity (µmol min−1 mg−1) LbADHK71E activity (µmol min−1 mg−1) % increase
2-Acetylpyridine 10 104.1 ± 1.7 125.5 ± 1.1 21
2-Hexanone 10 22.8 ± 0.7 27.9 ± 1.1 22
acetophenone 5 29.8 ± 0.7 39.5 ± 0.7 33
4-Hydroxy-2-butanone 10 39.5 ± 1.3 54.9 ± 4.6 39
  1. Mean values obtained from a single protein preparation and three technical replicates are shown