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Table 3 Kinetic parameters of purifed LbADHWT and LbADHK71E for various substrates

From: NADPH biosensor-based identification of an alcohol dehydrogenase variant with improved catalytic properties caused by a single charge reversal at the protein surface

Substrate Vmax (µmol min−1 mg−1) KM (mM)
LbADHWT LbADHK71E LbADHWT LbADHK71E
2,5-Hexanedionea 148.5 ± 12.3 173.3 ± 11.1 5.1 ± 0.6 4.3 ± 0.5
Methyl acetoacetateb 154.2 ± 2.2 221.2 ± 3.3 1.13 ± 0.06 0.67 ± 0.04
  1. aMean values and standard deviation based on three separate protein preparations with three technical replicates per preparation
  2. bMean values and standard deviation based on a single protein preparation and three technical replicates