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AMB Express

Table 3 Kinetic parameters of purifed LbADHWT and LbADHK71E for various substrates

From: NADPH biosensor-based identification of an alcohol dehydrogenase variant with improved catalytic properties caused by a single charge reversal at the protein surface

Substrate

Vmax (µmol min−1 mg−1)

KM (mM)

LbADHWT

LbADHK71E

LbADHWT

LbADHK71E

2,5-Hexanedionea

148.5 ± 12.3

173.3 ± 11.1

5.1 ± 0.6

4.3 ± 0.5

Methyl acetoacetateb

154.2 ± 2.2

221.2 ± 3.3

1.13 ± 0.06

0.67 ± 0.04

  1. aMean values and standard deviation based on three separate protein preparations with three technical replicates per preparation
  2. bMean values and standard deviation based on a single protein preparation and three technical replicates
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