Fig. 1

Location of the primers designed in this study with reference to ahy genes of D. oleovorans. The positioning of ahy primers refers to the Desulfococcus oleovorans strain Hxd3 ahy gene (WP_012173623.1). Initial primers are shown in red and improved primers are shown in pink. After being tested, primers ahyA1377F and ahyA1843R’ shown in red were selected for subsequent use