Strains names | Site | Types | Gene |
---|
Original E. coli | No genetic change |
LB-control | 1210–1215 | TTANGG | mdtF |
LB-control | 572 | G → A | vanRI |
Cu-H | 1203–1217 | GATAGGGTTANTGGC | mdtF |
Cu-L | 572 | G → A | vanRI |
Tet | 572 | G → A | vanRI |
Tet + (Cu-L) | 572 | G → A | vanRI |
Tet + (Cu-H) | 572 | G → A | vanRI |
- The mdtF is the multidrug inner membrane transporter for the mdtEF-TolC efflux complex; vanRI is the regulatory transcriptional activator in the vanSR regulator within the vanI glycopeptide resistance gene cluster. H: resistant strains selected at high copper ion exposure concentrations that gradually increased up to 1/10 MIC level; L: resistant strains selected at low mental ion exposure concentrations about 1/100 MIC