Seed treatment with prodigiosin controls damping-off of cucumber caused by Pythium ultimum

AMB Express

Table 3 Suppression of damping-off of cucumber caused by Pythium ultimum in soil-less mix with ethanol extract from Serratia marcescens strains N4-5 and Tn246 normalized on a total protein basis

Treatment^a	Mean plant stand per pot at different P. ultimum infestation levels^b
Treatment^a	0	10	30	50
Non-treated seed	4.84 AB	3.78 DEF	2.16 KLM	1.50 MN
Ethanol-only	4.94 A	3.63 EFG	2.56 I → L	1.13 N
1X N4-5 ethanol extract	4.59 A → D	4.25 A → E	3.50 E → H	3.81 C → F
1/3X N4-5 ethanol extract	4.88 AB	4.03 B → E	2.66 H → K	3.13 F→J
1/4X N4-5 ethanol extract	4.88 AB	4.66 A → D	3.44 E → I	2.88 G → K
Tn246 ethanol extract	4.68 ABC	3.41 E → I	2.44 JKL	1.75 LMN

^aSee “Materials and methods” for a description of treatments. Non-treated seed; no treatment was applied. Ethanol-only, seeds were treated with ethanol. N4-5 ethanol extract, seeds were treated with ethanol extract of strain N4-5. Tn246 ethanol extract, seeds were treated with ethanol extract of strain Tn246
^bValues were the mean of four experiments (n = 4) expressed as mean plant stand per pot. Results were combined prior to analysis as there was no significant experiment × treatment effect (P = 0.1898). Treatments in all experiments contained eight replicate pots, each containing five seeds. Numbers followed by the same letter were not significantly different (P ≤ 0.05) as determined by a protected least significant difference test. Least significant difference was 0.68. P. ultimum was added at 0, 10, 30, and 50 sporangia/cm³ soil-less mix inoculum