Fig. 5

Sensitivity analysis. Tenfold serial dilutions of the template (2.5 ng, 250 pg, 25 pg, 2.5 pg, 250 fg, 25 fg, 2.5 fg) or the pMD19-T plasmid(3.15 * 10⁶ copies, 3.15 * 10⁵ copies, 3.15 * 10⁴ copies, 3.15 * 10³ copies, 3.15 * 10² copies, 3.15 * 10 copies, 3.15 copies) were added to standard MCDA reactions and incubated at 61 °C for 1 h, respectively. Diagnosis techniques, including colorimetric indicator (a, c) and gel electrophoresis (b,d) were used for analysis of MCDA amplicons. Tubes (a)/Lanes (b) 1–8 respectively represent S. agalactiae strain ATCC12386 DNA levels of 2.5 ng, 250 pg, 25 pg, 2.5 pg, 250 fg, 25 fg, 2.5 fg per reaction, and a blank control (DW). Tubes (c)/Lanes (d) 1–8 respectively represent the pMD19-T plasmid levels of 3.15 * 10⁶ copies, 3.15 * 10⁵ copies, 3.15 * 10⁴ copies, 3.15 * 10³ copies, 3.15 * 10² copies, 3.15 * 10 copies, 3.15 copies per reaction, and a blank control (DW)