Table 1 ELISA results of HRP conjugated antibody
Diluted from 10 mg/ml | Antibody concentration (ng/ml) | HRP conjugated antibody | |
|---|---|---|---|
1 | 1:1000 | 10000.00 | 2.716 |
2 | 1:2000 | 5000.00 | 2.286 |
3 | 1:4000 | 2500.00 | 1.578 |
4 | 1:8000 | 1250.00 | 1.045 |
5 | 1:16,000 | 625.00 | 0.678 |
6 | 1:32,000 | 312.50 | 0.443 |
7 | 1:64,000 | 156.25 | 0.266 |
8 | 1:128,000 | 78.13 | 0.194 |
9 | 1:256,000 | 39.07 | 0.129 |
10 | 1:512,000 | 19.54 | 0.117 |
11 | Blank | Blank | 0.053 |
12 | Blank | Blank | 0.053 |
Titer | Titer | 1:512,000 |