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AMB Express

Table 1 The primers for the sequences of CAP and ΔCAP

From: High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli

Probe/primer

The DNA sequence (5′-3′)

Tm

Amplicon size (bp)

CAPF

tttt ggatcc ATGAGACACAGAGCTATATTCAG

55.40

650

ΔCAPF

tttt ggatcc CCCACAGCTGGCACATAC

58.09

554

CAPR

tttt ctcgag TTCACTTAGAGAACGGACTTG

55.31

 
  1. The lowercase letters are protective bases and restriction sites, respectively
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