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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: Gα3 subunit Thga3 positively regulates conidiation, mycoparasitism, chitinase activity, and hydrophobicity of Trichoderma harzianum

Fig. 1

Construction of thga3 deletion and complemented mutants. a Thga3 gene deleting strategy. b Thga3 gene complementing vector pKH-KO-Gthga3. c PCR and RT-PCR identification of thga3, hyg, and neo genes in Δthga3 and Rthga3. M, 250-bp ladder. PCR templates were genomic DNA and cDNA of wild-type Th33, genomic DNAs of Δthga3 and Rthga3. d Southern hybridization analysis. Probe A. Thga3 ORF; Probe B. Hyg gene; ProbeC. Neo gene. DNAs of wild-type Th33, Δthga3 and Rthga3 were digested by EcoRI/XhoI

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