Table 2 Phylogenetic affiliation of isolates on the basis of 16S rRNA gene sequences by using the BLAST program in the GenBank database based on sequence similarity and determination of toxin genes
No | Isolated | Genbank code | GenBank reference | Similarity (%) | Genes | |||
|---|---|---|---|---|---|---|---|---|
toxR | tdh | trh | Tlh | |||||
1 | Vibrio alginolyticus strain YHTH7 | MT953948 | MN874162.1 | 98.06 | – | – | + | – |
2 | Vibrio azureus strain YVL11 | MT953949 | KT986135.1 | 100 | – | – | – | – |
3 | Vibrio azureus strain HTH12 | MT953950 | KT986135.1 | 100 | – | – | – | – |
4 | Vibrio alginolyticus strain YN14 | MT953951 | MH298564.1 | 98.05 | + | – | – | + |
5 | Vibrio fluvialis strain YHTH16 | MT953952 | CP051126.1 | 100 | + | – | – | + |
6 | Vibrio alginolyticus strain YVL22 | MT953953 | MN843961.1 | 99.72 | + | – | + | + |
7 | Vibrio alginolyticus strain YVL24 | MT953954 | MN938185.1 | 99.86 | + | + | – | + |
8 | Vibrio alginolyticus strain YVL26 | MT953955 | CP051109.1 | 99.59 | – | – | – | – |
9 | Vibrio orientalis strain YVL27 | MT953956 | MN945276.1 | 100 | – | – | – | + |
10 | Vibrio alginolyticus strain YN34 | MT953957 | MN938360.1 | 99.65 | – | – | + | + |
11 | Vibrio azureus strain YVL5 | MT953958 | KT986135.1 | 100 | – | – | + | – |
12 | Vibrio azureus strain HTH6 | MT953959 | KT986135.1 | 100 | + | – | – | + |
13 | Vibrio fluvialis strain YHTH18 | MT953960 | CP051126.1 | 100 | + | – | + | – |
14 | Vibrio alginolyticus strain YN19 | MT953961 | MH298564.1 | 98.05 | + | – | – | + |
15 | Vibrio alginolyticus strain YN29 | MT953962 | MN843961.1 | 99.72 | – | – | – | + |
16 | Vibrio alginolyticus strain YVL31 | MT953963 | MN938185.1 | 99.86 | – | – | + | + |
17 | Vibrio alginolyticus strain YVL40 | MT953964 | CP051109.1 | 99.59 | + | – | – | + |
18 | Vibrio alginolyticus strain YVL43 | MT953965 | MN938360.1 | 99.65 | + | – | – | – |
19 | Vibrio orientalis strain YVL42 | MT953966 | MN945276.1 | 100 | + | – | + | + |
20 | Vibrio alginolyticus strain YHTH44 | MT953967 | MN874162.1 | 98.06 | + | – | + | + |
21 | Vibrio azureus strain YVL45 | MT953968 | KT986135.1 | 100 | + | – | – | + |
22 | Vibrio azureus strain YVL46 | MT953969 | KT986135.1 | 100 | – | – | + | – |
23 | Vibrio fluvialis strain YHTH47 | MT953970 | CP051126.1 | 100 | – | – | – | – |
24 | Vibrio azureus strain YVL33 | MT953971 | KT986135.1 | 100 | + | + | – | – |
25 | Vibrio azureus strain YHTH35 | MT953972 | KT986135.1 | 100 | – | – | – | + |
26 | Vibrio fluvialis strain YHTH37 | MT953973 | CP051126.1 | 100 | – | – | – | + |
27 | Vibrio alginolyticus strain YN38 | MT953974 | MH298564.1 | 98.05 | + | – | – | + |
28 | Vibrio alginolyticus strain YVL84 | MT953975 | MN843961.1 | 99.72 | – | – | – | – |
29 | Vibrio alginolyticus strain YVL85 | MT953976 | MN938185.1 | 99.86 | – | – | – | + |
30 | Vibrio alginolyticus strain YVL86 | MT953977 | CP051109.1 | 99.59 | + | – | – | – |