Establishment of the monomeric yellow-green fluorescent protein mNeonGreen for life cell imaging in mycelial fungi

AMB Express

Table 2 List of plasmids used in this study

Plasmid	Characteristics	Source
pRS-nat	amp^R, ura3, nat^R	(Klix et al. 2010)
p1783-1	amp^R, ura3, hyg^R, Pgpd::egfp::TtrpC	(Pöggeler et al. 2003)
pRHN1	amp^R, ura3, nat^R Pgpd::Dsred::Ttrpc	(Janus et al. 2007)
pDsRed-SKL	amp^R, nat^R Pgpd::Dsred-SKL::TtrpC	(Elleuche and Pöggeler 2008)
pDest::amp	amp^R	Dahlmann et al. 2020
pGG-C-F	amp^R,, nat^R Pgpd::3xFLAG::TtrpC	Dahlmann et al. 2020
pNpX-GFP	amp^R, ura3, nat^R Pgpd::egfp::TtrpC	(Bloemendal et al. 2014)
753-pENTR20_mNeonGreen-N1	kan^R, mng (optimized)	Schink, pers. communication
pxyl-mNeonGreen	amp^R, ura3, nat^R Pxyl::mng	This study
pxyl-mng	amp^R, ura3, nat^R Pxyl::mng::TtrpC	This study
pGG-C-F-mng	amp^R, nat^R Pgpd::mng::3xFLAG::TtrpC	This study
pmng-SKL	amp^R, ura3, nat^R Pgpd::mng-SKL::TtrpC	This study

nat^R: nourseothricin resistant, hyg^R: hygromycin resistant; amp^R: ampicillin resistance; ura3, Orotidine-5′-phosphate decarboxylase gene of S. cerevisiae; Pxyl: promoter of the xylose gene Smxyl of Sordaria macrospora; Pgpd: promoter of the glycerinaldehyd-3-phosphat-dehydrogenase-gene of Aspergillus nidulans; TtrpC: terminator of the anthranilat synthase gene of Aspergillus nidulans; ssi: single spore isolate; SKL: peroxisomal targeting sequence Ser-Arg-Leu; mng: gene for green fluorescence protein monomeric NeonGreen, (mNG) of Branchiostoma lanceolatum; Dsred: gene for red fluorescence protein (DsRed) of Discosoma species; egfp: gene for green fluorescence protein enhanced green fluorescent protein (eGFP) of Aequorea victoria