Establishment of the monomeric yellow-green fluorescent protein mNeonGreen for life cell imaging in mycelial fungi

AMB Express

Table 1 List of primers used in this study

Name	Sequence (5′–3′)
Pxyl_fw	^GACTGGTCTCA^AGTCGAACCTTCTCTTCTCCATTTCTT
Pxyl_rev	^CAGAGGTCTCA^GCAGGTTGGCGGTTTCTGGTTAGGCC
Neon_fw	^CAGAGGTCTCA^CTGCATGGTGAGCAAGGGCGAGGAGG
Neon_rev	^CTCAGGTCTCC^CGTACTACTTGTACAGCTCGTCCATGC
Pxylneon_fw	^{GTAACGCCAGGGTTTTCCCAGTCACGACG}GAACCTTCTCTTCTCCATTT
Pxylneon_rev	^{GCGGATAACAATTTCACACAGGAAACAGC}CTACTTGTACAGCTCGTCCA
trpC-neon	^{GCCTTTACCGATGTGATGGGCATGGACGAGCTTTACAAGTAG}AGCGGCCGCTCTAGAACTAGTGGATCCACT
TtrpC_pRS_r	^{GCGGATAACAATTTCACACAGGAAACAGC}TCGAGTGGAGATGTGGAGTGG
Neon_fw-2	^{CGAGTCAGGTCTCC}^TGGTATGGTGAGCAAGGGCGAG GAGG
Neon_rev-2	^{ACCGACAGGTCTCG}^ATCCCTTGTACAGCTCGTCCATGC
Neo_f	ATGGTGAGCAAGGGCGAG
Neo_SKL_TrpC_r	^{GTTTGATGATTTCAGTAACGTTAAGTGGA}*TTAGAGCTTGCT*CTTGTACAGCTCGTCCATGC
pRS_Pgpd_f	^{GTAACGCCAGGGTTTTCCCAGTCACGACG}GTACAGTGACCGGTGACTCT
Pgpd_Neo_r	^{ATGTTATCCTCCTCGCCCTTGCTCACCAT}GGGAAAAGAAAGAGAAAAGAAAAGAGCA
TtrpC_f	TCCACTTAACGTTACTGAAATCATCAAAC

bold superscript = overhang; superscript underlined = the four base pairs after the BsaI recognition site in the overhang, bold italics = tripeptide SKL and a stop codon
T terminator, P promotor