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Fig. 1 | AMB Express

Fig. 1

From: Establishment of the monomeric yellow-green fluorescent protein mNeonGreen for life cell imaging in mycelial fungi

Fig. 1

Physical maps of mNG plasmids. a pxyl-mng, b pGG-C-F-mng, c pmng-SKL The ampicillin resistance cassette (bla) and the ori for selection and replication in E. coli are indicated. The nourseothricin resistance gene nat1 is under control of the A. nidulans trpC promoter (PtrpC) can be used for selection of fungal transformants. The 2 µ ori and the ura3 gene can be used for transformation of S. cerevisiae. The mng ORF (green arrow) is either regulated by the xylose inducible Smxyl promoter of S. macrospora (Pxyl) or the gpd promoter A. nidulans (Pgpd) and the trpC terminator of A. nidulans (TtrpC). Plasmid pxyl-mng contains a multiple cloning site (MCS). In plasmid pGG-C-F-mng a C-terminal 3 × FLAG tag: (DYKDHD-G-DYKDHD-I-DYKDDDDK, red arrow) is fused via a short linker (GSGSG) to mNG. In plasmid pmng-SKL the peroxisomal targeting sequences SKL (orange arrow) is fused to the C-terminus of mNG

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