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Fig. 5 | AMB Express

Fig. 5

From: Isolation and characterisation of Methylocystis spp. for poly-3-hydroxybutyrate production using waste methane feedstocks

Fig. 5

Poly-3-hydroxybutyrate (PHB) production of new methanotroph isolates. a Preliminary experiment to test optimal PHB accumulation with type strain OBBP. Growth was measured during the pre-culture which grew supplemented with nitrate (NO-3) which was harvested after 96 h (indicated by and arrow) and resuspended in medium without nitrate. Samples with black symbols and control (grown with nitrate for 192 h) with empty symbol. b PHB accumulation over time during nitrate limited phase (n = 2). PHB production of new methanotroph isolates compared to PHB producing type strain M. parvus OBBP and PHB negative strain Methylococcus capsulatus Bath from two individual experiments (c and d). c Test of methanotrophic PHB production on biogas from AD unfiltered (AD3) and filtered (AD4). d Test of methanotrophic PHB production on biogas from three different landfills. OBBP served as PHB producing positive control of the type II methanotrophs and Bath as negative control. The bar chart shows PHB per cell dry weight accumulated in a 35 mL culture after 3 days growth followed by three days growth without nitrate. All samples n = 3 with error-bars representing SEM. Graphs made using GraphPad Prism

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