Fig. 3From: Isolation and characterisation of Methylocystis spp. for poly-3-hydroxybutyrate production using waste methane feedstocksPhylogenetic tree comparing isolated strains of Methylocystis species with related species and whole genome alignment of isolated strains with closest relatives based on 16S rRNA similarity. a The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. This analysis involved 12 nucleotide sequences. Codon positions included were 1st + 2nd + 3rd + Noncoding. Bootstrap method was used as test of phylogeny with 1000 number of Bootstrap Replications. There were a total of 1547 positions in the final dataset. Evolutionary analyses were conducted in MEGA X9. b/c) The alignment shows structural and single nucleotide similarity between isolated strains and the closely related strains they were compared to. With Average Nucleotide Identity of 94.96%, M. rosea BRCS1 had significant levels of structural and single nucleotide differences when compared to M. rosea GW6 with accession number CP034086.1. a There was significantly less structural and single nucleotide difference between M. parvus BRCS2 and M. parvus OBBP with Average Nucleotide Identity of 99.99% (b)Back to article page