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Fig. 3 | AMB Express

Fig. 3

From: Isolation and characterisation of Methylocystis spp. for poly-3-hydroxybutyrate production using waste methane feedstocks

Fig. 3

Phylogenetic tree comparing isolated strains of Methylocystis species with related species and whole genome alignment of isolated strains with closest relatives based on 16S rRNA similarity. a The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. This analysis involved 12 nucleotide sequences. Codon positions included were 1st + 2nd + 3rd + Noncoding. Bootstrap method was used as test of phylogeny with 1000 number of Bootstrap Replications. There were a total of 1547 positions in the final dataset. Evolutionary analyses were conducted in MEGA X9. b/c) The alignment shows structural and single nucleotide similarity between isolated strains and the closely related strains they were compared to. With Average Nucleotide Identity of 94.96%, M. rosea BRCS1 had significant levels of structural and single nucleotide differences when compared to M. rosea GW6 with accession number CP034086.1. a There was significantly less structural and single nucleotide difference between M. parvus BRCS2 and M. parvus OBBP with Average Nucleotide Identity of 99.99% (b)

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