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Table 2 Fermentation characteristic and microbial counts of the alfalfa silages (g/kg of DM except as noted)

From: Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing

 

Control

CEGA

CELP

GALP

DM g/100 g

33.5 ± 0.31c

35.9 ± 0.41a

35.0 ± 0.20b

36.2 ± 0.32a

pH

5.2 ± 0.18a

4.8 ± 0.04bc

4.7 ± 0.07c

4.9 ± 0.09b

WSC g kgDM-1

13.0 ± 0.73c

14.9 ± 1.06bc

13.5 ± 0.93a

13.8 ± 0.62b

Fermentation products

Ammonia (g kgDM-1)

7.8 ± 0.41a

4.6 ± 0.29d

5.3 ± 0.61b

5.0 ± 0.25c

Lactic acid (g kgDM-1)

30.7 ± 1.17d

36.9 ± 2.36c

51.2 ± 2.41a

45.5 ± 2.04b

Acetic acid (g kgDM-1)

12.6 ± 1.48c

38.3 ± 1.94a

17.5 ± 0.98b

18.4 ± 1.09b

propionic acid (g kgDM-1)

1.8 ± 0.07a

0.2 ± 0.01b

0.2 ± 0.06b

0.3 ± 0.01b

Butyric acid (g kgDM-1)

3.1 ± 0.23a

0.0 ± 0.00b

0.0 ± 0.02b

0.1 ± 0.04b

LAB(log cfu g−1 FM)

7.5 ± 0.16b

8.3 ± 0.14a

8.5 ± 0.08a

8.5 ± 0.12a

Nutrient composition

Crude protein

21.7 ± 0.09c

22.6 ± 0.25a

23.5 ± 0.59a

23.3 ± 0.02b

NDF

43.1 ± 1.70a

41.7 ± 1.86b

40.2 ± 2.05c

42.6 ± 1.43b

ADF

34.9 ± 0.56a

32.3 ± 0.98b

31.3 ± 1.59b

33.3 ± 0.36ab

Crude Fatty

4.3 ± 0.05a

4.3 ± 0.03a

3.7 ± 0.05b

3.7 ± 0.04b

  1. CON untreated silage, CEGA silage treated with cellulase plus ɑ-galactosidase, CELP silage treated with cellulase plus L. plantarum, GALP silage treated with ɑ-galactosidase plus L. plantarum
  2. a, b, cMeans with different superscripts in the same row differ (P < 0.05)