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Fig. 9 | AMB Express

Fig. 9

From: Screening and selection strategy for the establishment of biosimilar to trastuzumab-expressing CHO-K1 cell lines

Fig. 9

Analysis of biosimilar candidate to trastuzumab in cell culture supernatant by Western blotting. Cell culture supernatants of cells growing in chemically defined, protein-free medium and shaking culture were loaded in 12% SDS-PAGE/reducing conditions (a and b) and 7.5% SDS-PAGE/non-reducing conditions (c). Immunodetection of heavy chain (a) was performed using an AP-conjugated goat anti-human IgG (γ chain specific) antibody. Immunodetection of light chain (b) and antibody whole molecule (c) was performed using a HRP-conjugated goat anti-human kappa light chain antibody. 1: Pre-stained molecular weight marker (NEB, UK). 2: biosimilar to trastuzumab obtained from NS0 cells (2.5 μg) (named 5G4). 3: Commercial trastuzumab (2.5 μg). 4: Cell culture supernatant of 5B12 clone. 5: Cell culture supernatant of 1B6 clone. 6: Cell culture supernatant of 6C8 clone

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